Structure prediction of Stem Bromelain from pineapples (Ananas Comosus) using procaricain enzyme as a modelling template

نویسندگان

  • Fatahiya Mohamed Tap
  • Fadzilah Adibah Abd Majid
  • Nurul Bahiyah Ahmad Khairudin
چکیده

Bromelain is a general name for a family of sulfhydryl that can be found in the proteolytic enzyme group from pineapples (Ananas Comosus). In this study focuses on the prediction of three dimensional structure (3D) of bromelain using homology modelling by MODELLER. The amino acid sequence of bromelain was obtained from the NCBI database and the analysis was done using various bioinformatics tools. 1PCI was used as the target templates for bromelain structure prediction. The 1PCI was found to be the most suitable template for the prediction of the bromelain structure.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The protease bromelain breaks oral tolerance and promotes sensitization of mice in a adjuvant-free murine model of pineapple (A.comosus) anaphylaxis

Background Allergies represent a socioeconomic problem that has affected increasing world population. A range of respiratory allergens are themselves proteases or are associated with proteolytic activity. Bromelain is a mixture of cysteine proteases present in the leaves and fruit Ananas comosus, with several pharmacological applications and is widely used in the food industry. Cases of occupat...

متن کامل

Bromelain: An Overview

Bromelain is a crude extract from the fruit or stem of pineapple [Ananas comosus (Linn.) Merr.] plant. It consists of different closely related proteinases which are good antiinflammatory, antithrombotic and fibrinolytic agents. The active fractions have been characterized biochemically and found to be effective after oral administration. It has earned universal acceptability as a phytotherapeu...

متن کامل

Studies of stability and characterization this enzyme bromelain in pineapple (Ananas comosus)

Background Bromelain is the generic name given to the set of derived endopeptidases belonging to members of the Bromeliaceae family, which belongs to the pineapple (Ananas comosus), being able to break the peptide bond, separating proteins and amino acids [1]. Bromelain possesses a wide range of therapeutic benefit as property of facilitating digestion of proteins, meat softening, ability to fa...

متن کامل

Recovery of recombinant bromelain from Escherichia coli BL21-AI

In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombina...

متن کامل

Thermal stability of free bromelain and bromelain-polyphenol complex in pineapple juice

Pineapple (Ananas comosus), Bromeliaceae family, is a fruit grows in tropical countries including Malaysia. This fruit has several pharmacological benefits due to the presence of high concentration of bromelain (cysteine proteases). Condition of elevated temperature will induce deformation of enzyme and result in loss of activity. Sulfhydryl groups in cysteine proteases are readily to be oxidiz...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2016